-, Mol Gen Genet. Access everything in the JPASS collection, Download up to 10 article PDFs to save and keep, Download up to 120 article PDFs to save and keep. • Physarum is subcultured onto Physarum agar, and is incubated at room temperature or 25 C. To maintain viability, plasmodial To maintain viability, plasmodial Physarum should be subcultured weekly. One mutant may be defective in both protein and deoxyribonucleic acid synthesis, and another only in deoxyribonucleic acid synthesis. Temperature, humidit... Time lapse video showing about two and a half days growth of a plasmodium of physarum polycephalum, with one frame for every 30 seconds. [Modern research on the cell cycle and mitosis in the myxomycete Physarum polycephalum]. Molecular and General Genetics MGG, Vol. Temperature-sensitive mutants of the slime mould Physarum polycephalum. The physarum polycephalum in form of sklerotia was cultivated in petri dishes covered with 1,5 and 2% agar-agar and pour with some drops of water. -, Genet Res. Thin layer chromatogram of crude lipids from Physarum cells at various periods after temperature shift from 24 C to 40 C. Lipids in each lane were extracted from 6.4 × 10 6 cells. USA.gov.  |  1977 Dec;12(4):1109-20 In the temperature range around 10–30 °C, the plasmodium shows thermotaxis to the higher temperature. Photobiol. Tso WW, Mansour TE (1975) Thermotaxis in a slime mold, Physarum NLM 1978 Mar;43(3):449-59 1980 Aug;143(2):897-905. doi: 10.1128/JB.143.2.897-905.1980. Action spectrum was determined, which accords well with action spectrum for generation of super oxide anion. With a growing open access offering, Wiley is committed to the widest possible dissemination of and access to the content we publish and supports all sustainable models of access. JSTOR®, the JSTOR logo, JPASS®, Artstor®, Reveal Digital™ and ITHAKA® are registered trademarks of ITHAKA. Our online platform, Wiley Online Library (wileyonlinelibrary.com) is one of the world’s most extensive multidisciplinary collections of online resources, covering life, health, social and physical sciences, and humanities. Changes in the membrane and zeta potentials, which were induced by chemical stimuli, decreased when the temperature decreased below T c . J Bacteriol. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Full Title: Sensory fusion in Physarum polycephalum and implementing multi-sensory functional computation Authors: James G.H. Physarum polycephalum Temperature compensation Access options Buy single article Instant access to the full article PDF. Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Access supplemental materials and multimedia. Curr Genet. JSTOR is part of ITHAKA, a not-for-profit organization helping the academic community use digital technologies to preserve the scholarly record and to advance research and teaching in sustainable ways. The growth-defective mutants were examined for protein and deoxyribonucleic acid synthesis and for aberrations in mitotic behavior. Mutants of the plasmodial phase. J Comput Theore Nanosci 8(3): 383-390. With pH remaining constant, (a) the time required for fruiting varies directly with temperature -i.e., the higher the temperature the longer the time required for fruiting; (b) the percentage of cultures producing fruiting bodies varies inversely with temperature-i.e., the higher the temperature, the smaller the percentage fruiting. For terms and use, please refer to our Terms and Conditions Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in … Speed maximum and half period length of the shuttle stream of Physarum polycephalum on the clinostat; temperature 20.5 (±0.3)° C. Request Permissions. Wiley has partnerships with many of the world’s leading societies and publishes over 1,500 peer-reviewed journals and 1,500+ new books annually in print and online, as well as databases, major reference works and laboratory protocols in STMS subjects. The transition temperature was constant at 16 C when plasmodia had been cultured above 16 C, and decreased gradually as the culture temperature decreased below … Wiley is a global provider of content and content-enabled workflow solutions in areas of scientific, technical, medical, and scholarly research; professional development; and education. Wiley has published the works of more than 450 Nobel laureates in all categories: Literature, Economics, Physiology or Medicine, Physics, Chemistry, and Peace. Maximum temperature at which P. polycephalum will fruit is between 32.5⚬ and 35.0⚬C. When transferred to the restrictive temperature, two mutants immediately lysed, whereas the remainder slowed or stopped growing. The “acellular” moniker derives from the plasmodial stage of the life cycle: the plasmodium is a bright yellow macroscopic multinucleate coenocyte shaped in a network of interlaced tubes. have been studied, and from data derived from observations made on 2,030 cultures, the following conclusions are drawn: The factors of pH and temperature are closely related and interdependent. 3, we prepared two thermal conditions in one agar plate using Peltier devices. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised 3) Physarum polycephalum shows negative phototaxis against UV-blue light. Phototaxis in Physarum polycephalum 271 A tungsten 8V-30W microscope lamp (Olympus Co., Tokyo) was used to observe proto-plasmic … The slime mould, Physarum polycephalum, meets a Danish cousin, Badhamia - and gets seriously bullied. movement of Physarum polycephalum M Iima et al Studies of the phase gradient at the boundary of the phase diffusion equation, motivated by peculiar wave patterns of rhythmic contraction in the amoeboid movement of Printed in As stated before, pH values at this temperature A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia - Volume 15 Issue 1 - Jennifer Dee, Russell T. M. Poulter Skip to main content Accessibility help We use cookies to distinguish you from other users … J Bacteriol. The P. polycephalumcultured on the gel vividly about 12 hours later. The negative selectant, netropsin, was used to enrich for temperature-sensitive mutants among a population of mutagenized amoebae, and, separately, a nonselective screening method was used to isolate plasmodial temperature-sensitive mutants among clonal plasmodia derived from mutagenized amoebae. The interrelationships of the factors of temperature and pH upon the fruiting of Physarum polycephalum Schw. @article{osti_22391127, title = {Reversible logic gates on Physarum Polycephalum}, author = {Schumann, Andrew}, abstractNote = {In this paper, we consider possibilities how to implement asynchronous sequential logic gates and quantum-style reversible logic gates on Physarum polycephalum motions. Theory of time-resolved somatic complementation and its use to explore the sporulation control network in Physarum polycephalum. Of the two lytic mutants, one affected both amoebae and plasmodia, and the other affected plasmodia alone. In the Kateb and Costello study , a total of 87 compounds and 79 compounds were identified at the incubation temperature of 75 C and 30 C, respectively, from the plasmodia of P. polycephalum. HHS It was then put in a darkroom with a constant temperature of 20°C. Generally speaking, the necessity of light for fruiting cannot be dispensed with by increasing acidity nor by lowering the temperature. © 1939 Wiley 163, Issue. Read your article online and download the PDF from your email or your account. 1976 Aug 2;146(3):313-5 64, … Heat sensitive factor necessary for mitosis onset in Physarum polycephalum (temperature shift/heat shock/cycloheximide/ts mutant). The higher the temperature at which a plasmodium is maintained, the greater the acidity must be in order to produce fruiting. Mol Gen Genet. ©2000-2021 ITHAKA. -, Toxicol Appl Pharmacol. Temperature-sensitive mutants of Physarum polycephalum: viability, growth, and nuclear replication. Physarum polycephalum, an acellular slime mold or myxomycete, is a protist with diverse cellular forms and broad geographic distribution. Michel Wright, Yvette Tollon, Regulation of thymidine kinase synthesis during the cell cycle of Physarum polycephalum by the heat-sensitive system which triggers mitosis and S phase, Experimental Cell Research, 10.1016/0014-4827(79)90304-5, 122, 2, (273-279), (1979). 1980 Apr;1(3):203-8. doi: 10.1007/BF00390945. We believe that this mutant, MA67, is affected in a step in the nuclear replication cycle occurring late in G2. On the day before the measurement, dried P. polycephalumwas placed on an agarose gel and lightly sprayed with water to induce germination. This site needs JavaScript to work properly. Our core businesses produce scientific, technical, medical, and scholarly journals, reference works, books, database services, and advertising; professional books, subscription products, certification and training services and online applications; and education content and services including integrated online teaching and learning resources for undergraduate and graduate students and lifelong learners. Physarum polycephalum was cloned from a cDNA library, and the nucleotide sequence of the cDNA was determined (accession no. Lane 1 , 0 min after temperature shift; Lane 2 , 1 min after temperature shift; Lane 3 , 2 min after temperature shift; Lane 4 , 5 min after temperature shift; Lane 5 , 10 min after temperature shift. 1, p. 91. Molecular and General Genetics MGG, Vol. Cycloheximide resistance of Physarum polycephalum. The plasmodium of Physarum polycephalum is a unicellular and multinuclear giant amoeba that shows adaptive behaviors. 1976 Nov 24;149(1):115-9. doi: 10.1007/BF00275966. Eur J Biochem. All Rights Reserved. 163, Issue. Mol Gen Genet. COVID-19 is an emerging, rapidly evolving situation. The higher the pH value, the shorter the period of time a plasmodium will live. Founded in 1807, John Wiley & Sons, Inc. has been a valued source of information and understanding for more than 200 years, helping people around the world meet their needs and fulfill their aspirations. Action spectrum for sporulation and photoavoidance in the plasmodium of Physarum polycephalum, as modified differentially by temperature and starvation.  |  Sterilized whole meal oat flakes were served as food and positioned near of the slime mold. In the absence of oxygen, no negative AB259870). In the setup illustrated in Fig. With temperature a constant factor, (a) highest percentage fruiting is obtained at pH 3.0, gradually decreasing as the pH value is increased; (b) least time is required for fruiting at pH 4.0. American Journal of Botany Published by the Botanical Society of America continuously since 1914, the American Journal of Botany (AJB) is the Society’s flagship research journal. 1981 Dec;120(3):529-34. doi: 10.1111/j.1432-1033.1981.tb05732.x. -. Under the conditions of the above experiments, sclerotia cannot be produced at high temperatures (32.5⚬-35.0⚬) nor at low temperatures (8⚬-12⚬). 1977 Aug;131(2):473-6 Check out using a credit card or bank account with. -, Cell. The thermotaxis of the plasmodium is so strong and the organism without learning always proceeds to the higher temperature region. Variations in the surrounding temperature altered membrane activities at the critical temperature, T c =15 C in plasmodium of the true slime mold Physarum polycephalum. The latter shows a striking reduction in the frequency of postmitotic reconstruction nuclei at the restrictive temperature. Whiting 1, Ben P.J. This item is part of a JSTOR Collection. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways. 1975 Jun;62(6):283-9. doi: 10.1007/BF00608954. Characterization of thermosensitive mutants of Physarum polycephalum a plasmodial screening method for cell cycle mutants based upon synchronization at the restrictive temperature. 1, p. 1980;179(1):43-8. doi: 10.1007/BF00268444. Would you like email updates of new search results? II. Clipboard, Search History, and several other advanced features are temporarily unavailable. The petri dishes were wrapped in aluminum foil at room temperature (22-25°C) and sealed with parafilm. This same relationship exists for plasmodia maintained at low temperatures (8⚬-12⚬C.). Execution of this step is necessary for both mitosis and chromosome replication. Isolation of cell cycle mutants of Physarum polycephalum. Photochem. This stage of the life cycle, along with its preference for damp shady habitats, likely contributed to the original mischaracterization of the organism as a fungus. Laffler TG, Wilkins A, Selvig S, Warren N, Kleinschmidt A, Dove WF. Select the purchase Tanaka Y, Nakagaki T (2011) Cellular computation realizing intelligence of slime mold Physarum Polycephalum. option.  |  Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Regulation of protein synthesis in the plasmodial phase of Physarum polycephalum. Physarum polycephalum (Slime mold) Status Reviewed-Annotation score: -Experimental evidence at protein level i Function i Catalytic activity i Milk clotting activity. Since Howard stated that the optimum temperature for P. polycephalum is between 20.00 and 26.0?C., the first of the present series was conducted within this temperature range. Physarum polycephalum belongs to the Amoebozoa, the sister group to the Opisthokonts (i.e., fungi and animals) ( Cavalier-Smith 2003) which both together form the supergroup Amorphea ( … P. polycephalum is used as a model organ… AJB publishes peer-reviewed, innovative, significant research of interest to a wide audience of scientists in all areas of plant biology (e.g., biodiversity, structure, function, development, genetics, evolution, reproduction, systematics), all levels of organization (molecular to ecosystem), and all plant groups and allied organisms (cyanobacteria, algae, fungi, and lichens). Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways. Naturwissenschaften. de Lacy Costello 1,2, Andrew Adamatzky 1.1 Unconventional Computing Centre, University of the West of England, Bristol, UK. 1975 Dec;26(3):237-54 Please enable it to take advantage of the complete set of features! NIH Action Spectrum for Sporulation and Photoavoidance in the Plasmodium of Physarum polycephalum, as Modified Differentially by Temperature and Starvation Toshiyuki Nakagaki Corresponding Author Length of survival of plasmodia at high temperatures is dependent upon pH value and varies inversely with it. Physarum Polycephalum changes polyaniline properties Alice Dimonte 1, Tatiana Berzina 1 and Victor Erokhin 1 1 CNR-IMEM (National Council of the Researches ± Institute of Materials for Electronics and Magnetism), Parco Area delle Complementation in heterokaryons was used to sort the mutants into nine functional groups. History, and the nucleotide sequence of the West of England,,... Then put in a darkroom with a constant temperature of 20°C affected plasmodia alone in Physarum,! In mitotic behavior time a plasmodium will live your account 1977 Dec ; (!, please refer to our terms and conditions American Journal of Botany 1939! 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Library, and several other advanced features are temporarily unavailable: viability, growth, and another in... As food and positioned near of the factors of temperature and pH upon the fruiting Physarum... Mutants in two ways ITHAKA® are registered trademarks of ITHAKA Peltier devices, two mutants lysed! Diverse cellular forms and broad geographic distribution thermotaxis of the complete set of features a constant temperature of 20°C acidity. Maximum temperature at which P. polycephalum will fruit is between 32.5⚬ and 35.0⚬C computation. Online and download the PDF from your email or your account about 12 hours.! The gel vividly about 12 hours later Search results ):203-8. doi: 10.1111/j.1432-1033.1981.tb05732.x two ways ):237-54,... ):237-54 -, Toxicol Appl Pharmacol that shows adaptive behaviors decreased below T c varies inversely with it the! Fruit is between 32.5⚬ and 35.0⚬C fruiting can not be dispensed with by increasing acidity nor by lowering the at... Affected in a step in the plasmodial phase of Physarum polycephalum a screening. Screening method for cell cycle and mitosis in the membrane and zeta potentials, which were induced by chemical,... Forms haploid plasmodia, and nuclear replication cycle occurring late in G2 Buy single Instant. Latter shows a striking reduction in the nuclear replication cycle occurring late in G2 upon. Was then put in a step in the membrane and zeta potentials which! To take advantage of the plasmodium is so strong and the organism without always. J Comput Theore Nanosci 8 ( 3 ) Physarum polycephalum shows negative phototaxis against UV-blue light cell and! With by increasing acidity nor by lowering the temperature decreased below T c oxide anion aluminum foil at temperature...